The effect of cold pre-treatment period on callus formation of pepper (Capsicum annuum L.) anther culture of local Aceh perintis genotype

Abstract Plant tissue culture is effective for plant propagation or biotechnology purposes, one of which anther culture. Anther pepper difficult because callus growth, regeneration organs, and low effectiveness. Pre-treatment such as cold temperatures can increase the induction growth embryogenesis in cultures. This study aimed to determine effect pre-treatment period on formation local Aceh plants, Perintis genotype. The results showed that duration had no significant culture, based Anova analysis. highest percentage was found control, 8.3%. 48 hours 72 pre-treated obtained 6.6%, respectively. Meanwhile, lowest from 24-hour at 5%. According our result, anthers did not need be with it propagated directly.